OpenAccessBelV2, OpenAccess, Analysis, bibRecord, 000056

Heterotopic Bone Formation Around Vessels: Pilot Study of a New Animal Model

Identifieur interne : 000056 ( OpenAccess/Analysis ); précédent : 000055; suivant : 000057

Heterotopic Bone Formation Around Vessels: Pilot Study of a New Animal Model

Auteurs : Wei-Xin Cai ; Li-Wu Zheng ; Franz E. Weber ; Chun-Lei Li ; Li Ma ; Martin Ehrbar ; Roger A. Zwahlen

Source :

BioResearch Open Access [ 2164-7844 ] ; 2013.

RBID : PMC:3731688

Abstract

To achieve an easily established, safe, and reproducible animal model for the study of heterotopic bone formation around vessels, a small animal series using New Zealand White rabbits was performed. Three different dosages of recombinant human bone morphogenic protein (rhBMP-2) carried by fibrin matrix were tested. A guided tissue regeneration (GTR) membrane sheet was formed into a tube and allowed to harden; it served both to maintain the space around the vessel bundle and to separate the fibrin matrix with rhBMP-2 from skeletal muscle. Wrapped around the femoral vessel bundle and fixed in place, the tube was filled with the fibrin matrix containing rhBMP-2. The surgical site was closed in layers, and the postoperative healing was uneventful. All animals resumed their full preoperative daily activities 3–4 days after the operation. No adverse events such as wound dehiscence or infection occurred, and all animals could be sacrified at the scheduled date. Micro–computed tomography and histological investigations showed heterotopic bone formation around the vessel bundle in the medium- and high-dosage rhBMP-2 groups. An easy, safe, and reproducible animal model that allows the study of heterotopic bone formation around vessels was successfully established.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3731688

DOI: 10.1089/biores.2013.0025
PubMed: 23914333
PubMed Central: 3731688

Affiliations:

Links to Exploration step

PMC:3731688

Le document en format XML

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Heterotopic Bone Formation Around Vessels: Pilot Study of a New Animal Model

Heterotopic Bone Formation Around Vessels: Pilot Study of a New Animal Model

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Abstract

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